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RT-PCR was done using a standard SYBR-green PCR kit (SYBR Premix EX TaqTM II, Takara), and the gene-specific PCR amplification was conducted using the Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems, USA)

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Neuropharmacology, 121 , 7988

In future, more effective regimens should focus simultaneously on the epigenetic changes, inflammatory cytokines, abnormal wound-healing process, and balance between profibrotic and anti-fibrotic factors in order to provide promising options for this refractory disease